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Real-time PCR analysis of expression of mRNA for B7-H1, B7-H2, B7-H3, B7-DC, and GAPDH in BEAS2B cells (left, n = 6), epithelial cells from nasal scrapings (center, n = 8), and cultured primary nasal epithelial cells (right, n = 3). Values shown are mean ± SEM CT from Taqman analysis and reflect target mRNA. The Y scale is inverted so that higher bars represent higher levels of mRNA. Probes, primers and assays used are described in MATERIALS AND METHODS.
