Figure 2.

The extracellular domain of CAR is required for cell:cell localization and adhesion. (A) Immunofluorescence in CHO cells expressing CAR, CAR^D0, CAR^ΔD1, and CAR^ΔD2. Although each of the mutant forms is found at the cell surface, CAR is more often found limited to the junctions between cells expressing CAR (arrow). (B and C) Cell clustering of CHO cells expressing CAR, CAR^D0, CAR^ΔD1, or CAR^ΔD2. B represents clusters of > 3 cells and C represents clusters > 7. CAR showed significantly more and larger clusters than mutant forms of CAR (n = 3).