Figure 5.

Detection of cell-associated CXCL5 by immunocytochemistry in AEII cells. AEII cells were stimulated with LPS or unstimulated for 18 h before immunochemical staining with anti-CXCL5 or isotype-matched control Ab. Original magnification: ×1,000. CXCL5-producing cell (CPC) index was calculated as [immunofluorescence positive cells/total cells] × 100. A total of 3,000 cells were enumerated and percent positive cells were calculated. This is a representative result of four independent experiments.