Figure 6.

CXCL5 expression in the lung in response to LPS is regulated via the TLR4 signaling cascade. Mice were exposed to 300 μg of nebulized LPS for 20 min and the BALF and lungs were harvested at 2 h for CXCL5 detection. Minimal CXCL5 expression was detected in TLR4^−/−, MyD88^−/−, and TIRAP^−/− mice (A–E). Mice pretreated with the p38 inhibitor (M39) or the JNK inhibitor (SP600125) show attenuated expression of CXCL5 in BALF and lung homogenates (F–H). A total of six mice were tested in each group, and significance was calculated by a one-way ANOVA. Values that are significantly different between groups (P < 0.05) are indicated by asterisks.