Figure 5.

Effects of JAK inhibitors on IL-13–induced Gob5 expression by mouse primary TBE cells in culture. Mouse TBE cell cultures were performed as described in Figure 2. Before IL-13 (20 ng/ml) treatment, cultured cells were pretreated for 30 min with the JAK inhibitors, JAK inhibitor I (100 nM) and AG490 (10 μM), and RNA was isolated 24 h after IL-13 treatment. Relative fold increase of Gob5 message that is normalized with GAPDH is shown in the figure. n = 3; *P = 0.029; **P = 0.047.