Figure 6.

Western blot analysis of STAT6 phosphorylation in primary humanTBE cells after IL-13 treatment. Primary human TBE cells were treated with IL-13 at 20 ng/ml, as described in Figure 2. At various times after the treatment, cultures were harvested for protein extraction and Western blot analysis with both anti–phosphorylated STAT6 (upper blot) and anti–total STAT6 protein (lower blot) antibodies. A similar result has been repeated in another primary human TBE culture derived from a different donor (data not shown).