Skip to main content
. 2005 Aug 25;33(6):549–554. doi: 10.1165/rcmb.2005-0133OC

Figure 2.

Figure 2.

Immunoprecipitation of P-selectin from lung endothelial cells. IP, immunoprecipitate. SA-HRP, streptavidin–horseradish peroxidase. HV, high tidal volume. WB, whole blood perfusion; Rec-WB, reconstituted whole blood perfusion; DB, perfusion with leukocyte- and platelet- depleted blood. P-selectin was immunoprecipitated from lysates of primary endothelial isolates obtained from HV ventilated lungs. (A–D) SA-HRP blots show endothelial surface expression of P-selectin, under perfusion conditions of WB or DB in A and WB in C. Molecular weights are indicated on the left. Bars show densitometry. Gadolinium, 10 μM. Each bar: n = 3. Mean ± SE. *P < 0.05, compared with bar on left by paired t test.