TABLE 1.
Summary of lung engraftment after transplantation of bone marrow populations from donor sp-c–gfp reporter mice
| Cells Transplanted | No. of Recipients | Radiation Dose | Lung Injury | Engrafted AT2 cells out of no. of cells analyzed (FACS) |
Engrafted AT2 cells/sections analyzed (microscopy) |
|---|---|---|---|---|---|
| Whole marrow (107) | 3 | 11 Gy | No | 0 of 3 × 106 | 0/60 |
| Whole marrow (107) | 4 | 12 Gy | No | 0 of 4 × 106 | 0/80 |
| Whole marrow (107) | 9 | 14 Gy | No | 0 of 9 × 106 | 0/180 |
| Whole marrow (107) | 4 | 14 Gy | Bleomycin | 0 of 4 × 106 | 0/80 |
| HSCs (200) | 4 | 14 Gy | No | 0 of 4 × 106 | 0/80 |
| HSCs (200) | 4 | 14 Gy | Bleomycin | 0 of 4 × 106 | 0/80 |
| TOTAL | 28 | 0 of 28 × 106 | 0/560 |
Definition of abbreviations: FACS, fluorescence-activated cell sorter; GFP, green fluorescent protein; HSCs, hematopoietic stem cells; SP-C, surfactant protein C.