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The effect of differentiation, IFN-β, and IL-10 on CUG-binding protein. Undifferentiated THP-1 cells do not express CUG-binding protein (lane 1). PMA differentiation strongly induces CUG-binding protein (lane 2). Six hours after treatment with 1 U/ml of IFN-β, there is strong induction of CUG-binding protein (compare lane 3 to lane 4). IL-10 treatment did not produce significant alteration of CUG-binding protein in macrophages (lanes 5 and 6).
