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. 2005 Jul 7;33(5):438–446. doi: 10.1165/rcmb.2005-0103OC

Figure 4.

Figure 4.

Effects of PAH mutations on the colocalization of BMPR-II and c-Src tyrosine kinase in HEK293 cells. (A–C) HEK293 cells were transiently transfected with HA–c-Src tyrosine kinase and truncation mutant BMPR-II (K230fsX21), permeablilized, and stained with antibody against 6xHIS and FITC-conjugate anti-HIS antibody to visualize BMPR-II (K230fsX21) (green) and with antibody against HA and rhodamine-conjugated monoclonal anti-HA antibody to visualize c-Src tyrosine kinase (red). Merged image depicted lack of colocalization of BMPR-II (K230fsX21) with c-Src tyrosine kinase. (D–F) HEK293 cells expressing BMPR-II (R491W) point mutant and c-Src tyrosine kinase. Colocalization of BMPR-II point mutant and c-Src tyrosine kinase was found in the merged image (aggregates indicated by arrows). (G–I) Similar to the K230fsX21 BMPR-II mutant, lack of colocalization between BMPR-II and c-Src tyrosine kinase was also found with N861fsX10 BMPR-II mutant. Data were representative of three independent experiments.