Figure 5.

BMP-2, -4, and -7 stimulations inhibit c-Src phosphorylation (Tyr418). The human pulmonary smooth muscle cells were stimulated with 200 ng/ml of BMP-2, BMP-4, BMP-7 or without ligand as control for the indicated time periods. After ligand stimulation, cells were immediately fixed with 4% formaldehyde in PBS and assayed for the phosphorylation of c-Src (Tyr418). Results were normalized with the number of cells as described in MATERIALS AND METHODS. Data represented the mean ± SEM from two independent experiments with duplicates.