Figure 7.

Effects of BMPR-II mutations on c-Src phosphorylation (Tyr418). The human pulmonary arterial smooth muscle cells were transfected with BMPR-II wild-type or mutant (K230fsX21, N861fsX10, or R491W) expression constructs or empty vector (as control). Forty-eight hours after transfection, cells were treated with or without BMP-2 for 10 min. The cells were then fixed and assayed for c-Src phosphorylation (Tyr418). Results were normalized with the number of cells. Data represented the mean ± SEM from two independent experiments with duplicates.