TABLE 1.

Primers used in sequencing, cloning, and mutagenesis

Primer Name	Sequence
Matchmaker 5′AD Sequencing	CTATTCGATGATGAAGATACCCCACCAAACC
Matchmaker 3′AD Sequencing	GTGAACTTGCGGGGTTTTTCAGTATCTACGATT
Matchmaker 5′DBD Sequencing	TCATCGGAAGAGAGTAG
Matchmaker 3′DBD Sequencing	GTCACTTTAAAATTTGTAT
BMPR2 wild-type (1–18)	ATGACTTCCTCGCTGCAG
BMPR2 wild-type (3114–3096)	CAGACAGTTCATTCCTATA
BMPR2 (K230fsX21) forward	GTCCAGTTGCTGTAAATTGTTTTCCTTTGC
BMPR2 (K230fsX21) reverse	GCAAAGGAAAACAATTTACAGCAACTGGAC
BMPR2 (R491W) forward	CAGGATGCAGAGGCTTGGCTTACTGCACAG
BMPR2 (R491W) reverse	CTGTGCAGTAAGCCAAGCCTCTGCATCCTG
BMPR2 (N861fsX10) forward	GACACCCGGCTGAATATAATTCCAGTCCTG
BMPR2 (N861fsX10) reverse	CAGGACTGGAATTATATTCAGCCGGGTGTC