Figure 1.

Localization of RHAMM after intratracheal treatments. RHAMM distribution was determined using diaminobenzadine-based immunohistochemistry using R36. (a) Uninjured control lung section stained with R36 preincubated with recombinant RHAMM protein shows no staining, confirming antibody specificity. (b) Normal RHAMM expression (brown staining) is restricted to the apex of airway epithelial cells, resident alveolar macrophages, and in bronchiolar smooth muscle. (c) At 4 d after injury, accumulating macrophages stain strongly for RHAMM. Macrophages were identified using the cell-specific marker, ED1 (data not shown; see Ref. 36). Photomicrographs shown are representative of at least five animals examined at each time and for each condition.