Figure 1.
In vitro and in vivo characterization of a Gal− mutant of K. pneumoniae. (A) Appearance of wild-type and Gal− strains on MacConkey base agar containing 1% galactose 24 h after culture from a dually infected mouse lung. (B) Growth of the wild-type and Gal− strains in LB broth, where rates of proliferation were indistinguishable (results shown as means ± SD of three experiments, P = ns). (C) Seven-day survival curves for both strains after a 1,000-CFU intranasal inoculation (n = 10 animals per group, P = ns). (D) Comparable intrapulmonary burden of wild-type and Gal− strains at 24 and 48 h after inoculation when delivered as single-hit inocula (n = 5 per strain per time point, P = ns).