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. 2005 Jul 29;33(5):490–499. doi: 10.1165/rcmb.2005-0049OC

Figure 1.

Figure 1.

Expression of ErbB family members and ligands in nontransformed (C10, E10) and malignant (A5, E9) sister lung cell lines. Western blots of lysates (50 μg protein for growth factor receptors and 20 μg protein for growth factors) were probed with antibodies as indicated. Sizes of the bands shown are 170 kD (EGFR), 185 kD (ErbB2), 185 kD (ErbB3), 180 kD (ErbB4), 18 kD (TGF-α), and 44 kD (Hrg-α). P85 was used as a loading control for growth factor receptors (6% gel), and β-actin was used as a loading control for growth factors Hrg-α (12% gel) and TGF-α (4–20% gradient gel). An extended exposure of the blot was used to confirm the low level of expression of EGFR in A5 cells (left panel, second row).