Figure 3.

IL-1β alone increased chemokine secretion. The cells were cultured and harvested as stated in Figure 1. Varying concentrations of IL-1β were added on Day 7 of culture, and the media and cells were harvested 24 h later. IL-1β (100 ng/ml) increased the secretion of MIP-2, CINC-2, and MCP-1. The original values are ng/ml and have been normalized in each experiment to the value of the control without IL-1β for each phenotype and expressed as % of control. The results are the means ± SE of six independent experiments. The asterisk signifies an increase compared with the control without addition of IL-1β (P < 0.05).