Figure 4.

The cytokine mixture increased mRNA levels of CINC-1, CINC-2α, CINC-3, and MCP-1 but not CINC-2β. Type II cells were cultured as described in Figure 1, and the cytokine mixture (10 ng/IL-1β, 10 ng/ml TNF-α, and 10 ng/ml IFN-γ) was added on Day 7 of culture. The cells were harvested and the RNA was extracted 4 h later. The mRNA values were measured by quantitative real-time PCR as stated in MATERIALS AND METHODS. The results are the means ± SE for four independent experiments. The asterisk signifies an increase compared with the control without addition of cytokines, P < 0.05.