Figure 5.

The mRNA levels of CINC-2α and CINC-2β show different responses to cytokine stimulation. Differentiated and dedifferentiated cultures of alveolar type II cells were cultured as defined in MATERIALS AND METHODS. The cytokine mixture (10 ng/ml IL-1β, 10 ng/ml TNF-α, and 10 ng/ml IFN-γ) was added on Day 7 of culture, and 4 h later the mRNA was extracted and processed for Northern analyses. A shows results for CINC-2α and B shows results for CINC-2β. Cytokines increased mRNA levels for CINC-2α in both phenotypes. The estimated size for the single CINC-2α transcript is 0.8 kb. Cytokines did not increase the mRNA level for CINC-2β. The estimated sizes of the two transcripts for CINC-2β are 2.7 kb and 4.1 kb. The results are from one experiment but are representative of three independent experiments.