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Sinus gland extract (SGE) without the known neuropeptides caused no change in VtG mRNA in hepatopancreas of stage 3 in vitro. Hepatopancreas fragments were incubated with sinus gland extract from which CPRP, CHH, and MIH were removed (SGE(-)) at 25% and 5% sinus gland equivalent; CPRP HPLC fraction (CPRP F) at 25% and 5% sinus gland equivalent; and at 25 nM CPRP. Control level is indicated by a dashed line and data are presented as mean ± SEM (N = 5).
