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. Author manuscript; available in PMC: 2010 Jul 1.

Published in final edited form as: Microbes Infect. 2009 May 4;11(8-9):762–769. doi: 10.1016/j.micinf.2009.04.016

Fig. 3 — The majority of C3 fixation by FtN incubated in 50% PHS was antibody-dependent. (A) FtN were incubated for 30 min in 50% PHS that had been depleted of anti-FtN antibody (dPHS, see methods) and assayed as above for IgG (left) and C3 (right) binding. N = 3. Results are normalized to the values seen with FtN incubated in 50% PHS. (B) FtN was incubated in either 50% AGS or 40% PHS for 30 min and assessed for C3 binding (Because AGS had been dialyzed at the time of its collection to remove antibiotics, it had been diluted approximately 10%. Thus, 50% AGS was compared with 40% PHS). The rightmost sample was incubated in HI-PHS prior to incubation in AGS (FtN/HI-PHS + AGS). Results are normalized to the value seen with FtN incubated in 40% PHS. N = 3. Results are expressed as the mean ± SD. *, P < 0.05; ***, P < 0.001.

Fig. 3 — The majority of C3 fixation by FtN incubated in 50% PHS was antibody-dependent. (A) FtN were incubated for 30 min in 50% PHS that had been depleted of anti-FtN antibody (dPHS, see methods) and assayed as above for IgG (left) and C3 (right) binding. N = 3. Results are normalized to the values seen with FtN incubated in 50% PHS. (B) FtN was incubated in either 50% AGS or 40% PHS for 30 min and assessed for C3 binding (Because AGS had been dialyzed at the time of its collection to remove antibiotics, it had been diluted approximately 10%. Thus, 50% AGS was compared with 40% PHS). The rightmost sample was incubated in HI-PHS prior to incubation in AGS (FtN/HI-PHS + AGS). Results are normalized to the value seen with FtN incubated in 40% PHS. N = 3. Results are expressed as the mean ± SD. *, P < 0.05; ***, P < 0.001.