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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Nat Mater. 2009 Jun 21;8(8):659–664. doi: 10.1038/nmat2473

Figure 1. Cytocompatible click hydrogel formation reaction and kinetics.

Figure 1

(a) Click-functionalized macromolecular precursors undergo the [3+2] Huisgen cycloaddition to form a 3D ideal network hydrogel via a step-growth polymerization mechanism. (b) Rheology can be used to monitor dynamic network formation and indicates gelation within minutes and complete reaction occurring in less than one hour at 37 °C for a 13.5 wt% monomer solution. G’ is shown as closed circles, while G’’ are open circles. (c) A Live/Dead stain at 24 hours of 3T3s encapsulated within this material indicates a predominantly viable population (live cells are shown in green, while dead cells are red). Image represents a 200 µm confocal projection. Scale bar = 100 µm.