FIG. 3.

Oncolytic activity of Adeno-hTERT-E1A and ONYX-015. Cell lines seeded 24 hr earlier were infected with Adeno-hTERT-E1A or ONYX-015 at the indicated MOIs. Three or 6 days later the cells were stained with crystal violet to quantify remaining cells in each of the indicated human tumor cells lines: (A) U251, (B) A549, (C) MCF-7/4HC, and (D) MDA-MB-231. Data shown in (A–D) represent means + SD, based on n = 3 replicates, with the uninfected cell controls set to 100%. (E) The replication-deficient adenovirus Adeno-β-gal was used to assay the intrinsic adenoviral infectivity of each cell line, as determined by X-Gal staining 48 hr postinfection. β-Galactosidase activity was quantified with a microtiter plate reader (A₆₅₀) after eluting the X-Gal stain in 1 ml of dimethyl sulfoxide per well. Data shown represent A₆₅₀ values normalized to crystal violet staining (means + SD, n = 2).