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. 2008 Dec 5;19(12):1383–1399. doi: 10.1089/hum.2008.056

FIG. 5.

FIG. 5.

Adenoviral replication and spread. Left: Hexon immunohistochemical staining (IHC) of A549 cells (A) or U251 cells (B) either 3, 5, and 7 days after infection (A) or 1, 3, and 5 days after infection (B) with either Adeno-βgal, Adeno-hTERT-E1A, or ONYX-015 as indicated, at an MOI of 3. Right: Supernatants from each well of an experiment similar to that shown on the left side of (A) and (B) were harvested and serial dilutions were applied to fresh 293 cells for 40 hr followed by hexon staining to titer functional adenovirus released from the initially infected cells (log scale, base 10). The decreases in A549 cell hexon staining on days 5 and 7 and U251 hexon staining on days 3 and 5 reflect massive cell death induced by ONYX-015 and Adeno-hTERT-E1A, respectively. (C) Left: Hexon staining of A549 cells 30 hr postinfection with either Adeno-βgal, Adeno-hTERT-E1A, or ONYX-015 at MOIs of 1 and 3. Right: Corresponding samples, in which cell lysates were applied to A549 indicator cells, which were stained for hexon protein 30 hr later. Adeno-β-gal is replication defective and serves as a negative control. All images were taken at a magnification of ×40.