Table 1.
Antibodies used for immunohistochemical studies
| Antibody | Clone | Source | Pre-treatment | |
|---|---|---|---|---|
| CD34 | Mouse anti-human IgG1/κ | QBEnd 10 | Dako, Carpinteria, California | None |
| CD99 | Mouse anti-human IgG1 | O13 | Signet, Dedham, Massachusetts | PC |
| Bcl-2 | Mouse anti-human IgG1/κ | 124 | Dako | PC |
| Epithelial membrane antigen (EMA) | Mouse anti-human IgG2a/κ | E29 | Dako | None |
| α-smooth muscle actin (SMA) | Mouse anti-human IgG2a | 1A4 | Sigma, St. Louis, Missouri | None |
| S-100 protein | Rabbit anti-human Ig | Polyclonal | Dako | None |
| Cytokeratin | Mouse anti-human IgG1/κ | AE1/AE3 | Dako | Pr |
PC pressure cooker antigen retrieval in 10 mM citrate buffer pH6.0, Pr digestion with 0.1% protease from Bacillus licheniformus (10 min at 37°C)