Fig. 1.
MiR-21 expression in human lung cancer cell lines. (A) MiR-21 expression levels were analyzed by qRT-PCR and expressed relative to hTERT-immortalized normal human bronchial epithelial cells (HBET2) (defined as 1.0, not shown). Data were mean ± SD from 3 independent experiments. The suppressive effects of AG1478 on cell growth were determined by MTS assay and indicated as IC50. *, P < 0.05 when compared with HBET2, Student's t-test. Ad, adenocarcinoma; La, large cell carcinoma; Mt*, EGFR mutant ΔE746-A750 (in-frame deletion of codons 746 to 750); Mt**, L858R (substitution from leucine to arginine at codon 858) and T790M (substitution from threonine to methionine at codon 790); Mt***, L858R; N/A, information not available; N, derived from never-smoker cases; S, derived from smoker cases; Sq, squamous cell carcinoma; Wt, EGFR wild-type. (B) Correlation between miR-21 expression and p-EGFR levels (Pearson's correlation, r = 0.71, P < 0.05). The miR-21 data were from panel A, and the p-EGFR data were obtained by quantitatively analyzing the results shown in Fig. S3.