Fig. 1.

Activation of wild-type and mutant V2R by peptidic and nonpeptidic agonists. (A) Intrinsic activity of V2R mutants in NDI. COS-M6 cells transiently expressing WT-V2R-GFP or its mutants were treated for 10 min. with 1 μM dDAVP or 1 μM VA88, (B) washed, lysed, and cAMP levels measured as described above. Untreated samples were set to 1 and significantly-increased cAMP levels (P < 0.05; student's t test) compared to mock-transfected cells are indicated by an asterisk. In parallel, cell equivalents were analyzed by immunoblotting. Protein masses are indicated in kDa.