Figure 4.

Expression of B, CrtL-b, and Pds in fruits and flowers of tomato. The relative amount of mRNA of B, CrtL-b, and Pds was measured concomitantly by RT-PCR from total RNA isolated from different stages of fruit ripening and flower development of wild-type (WT) L. esculentum (M82) and from the mutant Beta. PCR-amplified DNA fragments were separated by PAGE and autoradiographed. (A) WT fruits. (B) Beta fruits. (C) Flowers of wild type and B. Fruit ripening stages: G, mature green; B, breaker; BE, early breaker; BL, late breaker; O, orange; P, pink; R, red; Rx3 and Rx1/3 are samples that contained three times or one-third the total RNA from red fruit. L, leaf. Flowering stages: Fy, preanthesis flower; Fo, postanthesis flower.