Fig. 5.

The Toll pathway is a major target of CHMP2B^Intron5 toxicity. (A) The levels of the Toll receptor and Spaetzle precursor were increased in head homogenates of flies expressing CHMP2B^Intron5, CHMP2B^Intron5 and Spn5, or Shrub-GFP. The bracket indicates other possible Spaetzle precursor forms recognized by this antibody as reported by Chang and Morisato (36). Values on right are molecular mass in KD. The Western blot to detect Spaetzle was overexposed to show its C-terminal fragment more clearly. Quantification for the relative levels of Spaetzle precursor was done on lighter exposures from 3 independent experiments. (B) One copy of the spz² and spz^KG05402 mutant alleles partially suppresses the CHMP2B^Intron5 phenotype, and one copy of cact¹, cact⁴, or cact^KG07677 mutant alleles enhances the CHMP2B^Intron5 phenotype. (C) Extent of suppression or enhancement of the phenotype. n is the number of flies examined per genotype. (D) qRT-PCR analysis of the levels of drosomycin transcripts in fly head homogenates with different genotypes.