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. 2009 Jul 6;106(29):12043–12048. doi: 10.1073/pnas.0902449106

Fig. 3.

Fig. 3.

AML1-ETO expression induces a preleukemic state. (A–D) lz-gal4, UAS-gfp third instar wandering larvae of the indicated genotypes were bled and the absolute number of GFP+ cells (A and C) as well as the proportion of LZ-GFP+ cells expressing the crystal cell differentiation marker PO45 (B and D) were determined. (A) AML1-ETO but not AML1∂ETO or ETO induces a net increase in LZ-GFP+ circulating blood cells as compared to control larvae. (B) In the presence of AML1-ETO, the ratio of progenitors (GFP+, PO45−) to differentiated (GFP+, PO45+) crystal cells is almost inverted. Neither AML1∂ETO nor ETO affects this ratio. (C and D) Switching off the expression of AML1-ETO at the early L3 stage does not suppress the increase in circulating LZ-GFP+ cells (C) at the wandering larvae stage but partially restore crystal cell differentiation (P < 0.001) (D). **, significant difference (Student's t test, P < 0.001) compared to the wild-type strain.