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. 2009 Jul 6;106(29):12043–12048. doi: 10.1073/pnas.0902449106

Fig. 5.

Fig. 5.

Calpain inhibition reduces the clonogenicity of Kasumi-1 cells. (A) Relative viability of Kasumi-1 cells treated with increasing doses of ALLN or calpain inhibitor III (Inh III). (B) Relative colony numbers obtained upon treatment of Kasumi-1 or HL-60 cells with 10 μM ALLN or 30 μM Calpain Inhibitor III. (A and B) Significant differences between control and treated cells are indicated: *, P< 0.01; **, P < 0.001. (C) Colony forming activity and differentiation potential of primary blood cells treated with 10 μM ALLN or 30 μM calpain inhibitor III. B/CFU-E, blast/colony-forming unit erythroid; M, macrophage; G, granulocyte; GM, granulocyte-macrophage; and GEMM, granulocyte-erythrocyte-macrophage-megakaryocyte. (D) Western blots showing AML1-ETO or GAPDH expression in Kasumi-1 cells treated with 10 μM ALLN or 30 μM calpain inhibitor III. The relative levels of AML1-ETO (normalized to GAPDH) are indicated in the lower part of the panel.