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. 2009 Jul 1;106(29):12037–12042. doi: 10.1073/pnas.0903869106

Fig. 3.

Fig. 3.

DSB repair by an SSA-like mechanism and NHEJ. (A) The indicated strains (YW465, SAY1105, SAY1103, SAY1193, SAY1362, SAY1198, SAY1124, SAY1104, and SAY1199) were transformed with a plasmid construct containing a DSB flanked by 15-bp complementary overhangs embedded within an ADE2 gene. Repair efficiency was assessed by counting white colonies (Ade+) growing on medium lacking uracil and normalized to parallel transformations with supercoiled pRS413 and growth on medium lacking histidine. Transformation efficiency of each mutant strain is shown as a percentage of WT, which was defined as 100%. The standard deviation of 3 separate experiments is included. (B) GAL-HO assay of 10-fold serial dilutions showing cell survival during continuous HO expression and DSB induction at MAT using the indicated strains (JKM115, SAY272, SAY264, and SAY274). (C) NHEJ efficiency of the indicated strains (YW465, SAY1103, SAY1105, SAY1198, and SAY1104) is shown in a plasmid-rejoining assay of XhoI-linearized pRS416. Values for individual strains are plotted as a relative percentage of WT and include the standard deviation of 3 separate experiments.