Table 1.
EC50 values (μM), with SEM values in parentheses
| Site | Residue | EC50 |
|---|---|---|
| M2 receptor | ||
| W3.28 | –* | 1,900 (80) |
| W6.48 | Trp | 310 (6) |
| F2Trp | 1,100 (70) | |
| F3Trp | 420 (30) | |
| D2 receptor | ||
| F3.28 | Phe | 55 (1) |
| F1Phe | 140 (10) | |
| F2Phe | 36 (1) | |
| F3Phe | 140 (10) | |
| Cha | 97 (2) | |
| F5.47 | Cha | 78 (1) |
| F6.51 | Phe | 64 (4) |
| F1Phe | 76 (6) | |
| F2Phe | 4,200 (350) | |
| F3Phe | 6,200 (400) | |
| CNPhe | 1,340 (160) | |
| MePhe | 690 (40) | |
| Me2Phe | 75,000 (5,000) | |
| Cha | 55,000 (4,000) | |
| W7.40 | Trp | 190 (20) |
| F1Trp | 240 (9) | |
| F2Trp | 1000 (80) | |
| F3Trp | 170 (10) | |
| W6.48 | Trp | 42 (4) |
| F1Trp | 120 (10) | |
| F2Trp | 290 (30) | |
| F3Trp | 840 (60) | |
| F4Trp | 1,800 (300) | |
| Nap | 190 (20) | |
| F6.52 | Phe | 45 (3) |
| F1Phe | 41 (2) | |
| F2Phe | 1,700 (100) | |
| F3Phe | 5,500 (400) | |
| CNPhe | 240 (30) | |
| MePhe | 91 (6) | |
| Me2Phe | 33,000 (3,000) | |
| BrPhe | 1,500 (100) |
Hill coeffiecients generally range from 0.9 to 1.1; number of cells is generally >7. Full data are given in Table S1.
*Experiment with unacylated tRNA.