Table 1.
De-etiolation phenotype in the T-DNA insertion line SALK_024018
| oep16.1–1 | det-p, % | n | |
|---|---|---|---|
| Col-0 | wt | 14.7 | 265 |
| flu | wt | 31.6* | 196 |
| F6–4a | ho | 70.7* | 215 |
| 5.2 | ho | 85.8* | 190 |
| 5.10 | ho | 49.2* | 130 |
| 4.1 | ho | 15.0 | 233 |
| 4.2 | wt | 46.9* | 177 |
| 19.3 | wt | 48.4* | 182 |
| 2.2 | wt | 5.1 | 217 |
Except Col-0 and flu, all plants are progeny of the T-DNA insertion line SALK_024018 (see Fig. S2). F6–4a was published as Atoep16–1 by Reinbothe and coworkers (25). The de-etiolation phenotype (det-p) was monitored in 3 independent experiments on seedlings grown for 2.5 days in darkness. Three days after transfer to continuous white light (350 μmol · m−2 · sec−1), bleached, dead seedlings (compare Fig. 1) were quantified in percentage of all plantlets. wt, wild type for the oep16.1–1 allele; ho, homozygous for oep16.1–1; n, number of seedlings monitored in at least 3 independent experiments.
*, Lines with >30% dead seedlings (compare heterozygous flu control) were considered to show a de-etiolation phenotype. In parallel, all lines were PCR genotyped for the oep16.1–1 T-DNA insertion.