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. 2009 Jul 2;106(29):12061–12066. doi: 10.1073/pnas.0905441106

Fig. 5.

Fig. 5.

TLR-independent activation of DCs. (A) DC activation with cytoplasmic dsRNA and 5′ (3P)-RNA. Bone marrow (BM)-derived wt, 3d, and MyD88−/−/Trif−/− double-knockout DCs were transfected with 250 μg/mL poly(I:C) alone or by Lipofectamine with 10 μg/mL poly(dT:dA*dA:dT) or 200 ng 5′ (3P)-dsRNA. Poly(I:C) in high concentrations directly enters cells. P < 0.009 for all wt, 3d, and MyD88−/−/Trif−/− double-knockout DCs transfected with RNA versus Lipofectamine-treated (Lipo) DCs. (B) DC activation with cytoplasmic double-stranded mammalian DNA. DCs from wt, 3d, and TLR9−/− mice were transfected with 10 μg/mL calf thymus DNA. P < 0.05 for dsDNA-transfected wt, 3d, and TLR9−/− DCs versus Lipo alone DCs. A and B are mean ± SEM of triplicates.