Fig. 3.

Full purification of hOR17-4 from 2.65 L of bioreactor cultured cells. (A) SEC on immunoaffinity-purified hOR17-4. Absorbance was recorded at 280 nm (black line), 254 nm (gray line), and 215 nm (dashed line). Peaks 1–5 (indicated by numbers) were pooled and concentrated. The predicted monomer peak was pooled into an early fraction (peak 4) and a late fraction (peak 5). Peak 6 contains the 9-aa elution peptide TETSQVAPA from the immunoaffinity purification. (B) Total protein staining of SEC peak fractions. Column fractions were collected and subjected to SDS/PAGE followed by staining with Sypro Ruby. Load is the original immunopurified sample applied to the chromatography column. Peak numbers refer to those designated in A. Peaks 4 and 5 contain monomeric hOR17-4 at >90% purity.