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. Author manuscript; available in PMC: 2010 Jan 30.
Published in final edited form as: Mol Cell. 2009 Jan 30;33(2):237–247. doi: 10.1016/j.molcel.2008.12.026

Figure 2.

Figure 2

Down-regulation of B-RAF signaling activates AMPK. (A) Knockdown of B-RAF expression by RNA interference activates AMPK. SK-Mel-28 cells were infected with retrovirus containing two different shRNA constructs in pSUPER-retro against B-RAF or pSUPER-retro empty vector.

(B) Knockdown of MEK1 expression by RNA interference activates AMPK. SK-Mel-28 cells were infected with retrovirus containing two different shRNA constructs in pSM2C against MEK1 or control empty vector.

(C) Induction of AMPK phosphorylation by various inhibitors against the RAF-MEK-ERK signaling cascade. SK-MEL-28 cells were treated with DMSO, 20 μM U0126, or 50 μM PD98059 for 1 hr.