FIG. 2.
Bacteriostatic or bactericidal nature of nukacin ISK-1. CFU were counted (A) and optical density (OD) determined (B). The indicator bacterial strain L. sakei JCM 1157T was grown to the mid-log phase, and the culture was divided into three aliquots. Control cells (•) were not treated, and cells in the other two aliquots were treated with either nukacin ISK-1 (○) or nisin (▴). The lantibiotics (10× MIC) were added, the turbidity (OD) measured, and the CFU determined by the spread plate method after plating the cells on MRS agar in 10-fold serial dilutions. The arrow indicates the point at which nukacin ISK-1/nisin was added. This experiment was performed twice with high reproducibility. Representative data were used for the figure.