TABLE 1.

Nucleotide primers used in this work for detection of β-lactamase genes and their linkages with insertion sequences in A. baumannii isolates

Primer	Sequence (5′-3′)a	Target(s)	Expected amplicon size (bp)	Conditions for denaturation (°C/min)b	Conditions for annealing (°C/min)b	Conditions for extension (°C/min)b	Source or reference	Positive control (gene) [source or reference]
IMP-DIA-fwd	GGAATAGAGTGGCTTAATTCTC	blaIMP alleles	361	94/1	50/1	72/1	13	A. baumannii AC-54/97 (blaIMP-2) [25]
IMP-DIA-rev	GTGATGCGTCYCCAAYTTCACT
VIM-DIA-fwd	CAGATTGCCGATGGTGTTTGG	blaVIM alleles	523	94/1	50/1	72/1	26	P. aeruginosa VR143/97 (blaVIM-1) [18]
VIM-DIA-rev	AGGTGGGCCATTCAGCCAGA
SIM1-F	TACAAGGGATTCGGCATCG	blaSIM-1	570	94/1	50/1	72/1	19	A. baumannii YMC 03/9/T104 (blaSIM-1) [19]
SIM1-R	TAATGGCCTGTTCCCATGTG
OXA23-F	GATGTGTCATAGTATTCGTCG	blaOXA-23 alleles	748	94/1	50/1	72/1	2	A. baumannii Ab13 (blaOXA-23) [10]
OXA23-R	TCACAACAACTAAAAGCACTG
OXA24-F	TTCCCCTAACATGAATTTGT	blaOXA-24 alleles	582	94/1	50/1	72/1	2	A. baumannii RYC 52763/97 (blaOXA-24-type) [6]
OXA24-R	GTACTAATCAAAGTTGTGAA
OXA-58_I_Fw	GCTGAGCATAGTATGAGTCG	blaOXA-58 alleles	691	94/1	48/1	72/1	This work	A. baumannii VA-900/05 (blaOXA-58) [this work]
OXA-58_I_Rev	AAGCAAATGCCACCACTTGC
OXA-69A	CTAATAATTGATCTACTCAAG	blaOXA-51 alleles	975 or 2,168c	94/1	48/1	72/2	16	A. baumannii VA-804/03 (ISAba1 + blaOXA-90) [this work]
OXA-69B	CCAGTGGATGGATGGATAGATTATC
ISAba2_Fw	CCTTATCCTATCAGGGTTCTG	ISAba1 and blaADC alleles	2,300	94/1	50/1	72/2	This work	A. baumannii 16x46 (blaADC-type) [this work]
AmpCaba_Rev	GCATTCAGCACAGCATAAG

^aFor degenerate primers, the following code was used: Y, C/T.

^bAll reactions included an initial denaturation step of 5 min at 94°C, 30 cycles of amplification, and a final extension step of 20 min at 72°C.

^cPrimers give a larger amplicon in the presence of an ISAba1 upstream of the bla_OXA-51 allele.