TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Reference |
|---|---|---|
| Strains | ||
| N315 | Pre-MRSA strain carrying a functional mecI encoding mecA gene repressor, hetero-Metr, Nagasaki, Japan | 10 |
| N315P | Penicillinase plasmid-free strain derived from N315 | 10 |
| ΔIP | mecI null mutant derived from N315P (mecI::tetL), hetero-Metr Tetr | 15 |
| H14(or IPM8-14) | Mutant derived from ΔIP by selection with 8 mg/liter of IPM, VraS (S329→L) homo-Metr Tetr | 12 |
| ΔIP-KVR | vraSR null mutant from ΔIP (vraSR::cat), Mets Tetr Chlr | 12 |
| H14-KVR | vraSR null mutant from H14 (vraSR::cat), Mets Tetr Chlr | 12 |
| ΔIP-rifR | Mutant derived from ΔIP by selection with 1 mg/liter of RIF, RpoB (H481→Y) hetero-Metr Tetr Rifr | This study |
| ΔIP::vraSH14 | ΔIP in which vraS is replaced by vraSH14 (S329→L), homo-Metr Tetr | This study |
| ΔIP-rifR:: vraSH14 | ΔIP-rifR in which vraS is replaced by vraSH14, homo-Metr Tetr Rifr | This study |
| Mu3 | hVISA clinical strain, Tokyo, Japan | 9 |
| Mu50 | VISA clinical strain, Tokyo, Japan | 9 |
| NJ | VISA clinical strain, New Jersey | 23 |
| Plasmids | ||
| pKOR1 | S. aureus allele replacement vector | 2 |
| pKO- vraSH14 | pKOR1 harboring 1.0-kb PCR product of vraSH14 | This study |
a
Abbreviations: Mets, methicillin susceptible; Metr, methicillin resistant; Tetr, tetracycline resistant; Chlr, chloramphenicol resistant; Ampr, ampicillin resistant; Rifr, RIF resistant. Strains obtained by antibiotic selection are denoted “mutant.”