TABLE 1.
Primers used in this study
| Gene | Primer | Sequence (5′→3′) | Size of the amplified product (bp) |
|---|---|---|---|
| For PCRa | |||
| oqxA | oqxAF | CTCGGCGCGATGATGCT | 392 |
| oqxAR | CCACTCTTCACGGGAGACGA | ||
| oqxB | oqxBs | TTCTCCCCCGGCGGGAAGTAC | 512 |
| oqxBa2 | CTCGGCCATTTTGGCGCGTA | ||
| For real-time RT-PCR | |||
| adk | adkKF | ATGCGTATTATTCTGCTTGGCGC | 105 |
| adkKR | CAGCATATCGCCGGTGGAGAT | ||
| oqxB | oqxBKF | TCCTGATCTCCATTAACGCCCA | 131 |
| oqxBKR | ACCGGAACCCATCTCGATGC |
a
The PCR conditions for oqxA were 94°C for 45 s, 57°C for 45 s, and 68°C for 60 s with a cycle number of 34, and those for oqxB were 94°C for 45 s, 64°C for 45 s, and 72°C for 60 s with a cycle number of 32.