FIG. 3.

High-performance liquid chromatography analysis of peptidoglycan precursors of H. pylori J99 in the absence (top) and presence of pyrazolopyrimidinediones (HTS hit [middle] and compound D [bottom]). Elution times of N-acetyl-muramic acid (A), N-acetyl-muramic acid-alanine (B), N-acetyl-muramic acid-alanine-glutamate (C), N-acetyl-muramic acid-alanine-glutamate diaminopimelic acid (D), and N-acetyl-muramic acid-alanine-glutamate diaminopimelic acid-alanine-alanine (E) peptidoglycan precursors (Fig. 1) are indicated.