TABLE 3.

Relative amounts of UDP-MurNAc-tripeptide and -pentapeptide in E. coli OC2530 overexpressing MurF^a

Strain and compoundb	Amtc		UDP-MurNAc-tripeptide/UDP-MurNAc-pentapeptide ratio
	UDP-MurNAc-tripeptide	UDP-MurNAc-pentapeptide
E. coli OC2530/pASK
None (control)	63 ± 4	880 ± 17	0.07
DQ1	1,196 ± 71	213 ± 18	5.6
Cycloserine	2,255 ± 750	146 ± 3	15
E. coli OC2530/pMurF
None (control)	220 ± 65	2,674 ± 696	0.08
DQ1	933 ± 115	351 ± 0	2.7
Cycloserine	5,087 ± 494	181 ± 4	28

^aThe results are representative of those from two independent experiments.

^bEach of the compounds was used at 2× MIC.

^cAmounts are mAU of the high-pressure liquid chromatography peak area.