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. 2009 Jun 8;77(8):3244–3248. doi: 10.1128/IAI.00395-09

FIG. 1.

FIG. 1.

(A) Physical map of pMW1628 and gene maps of the pld locus of the wild-type Evir strain (top) and the Δpld mutant (bottom). Restriction sites and primer locations are indicated. (B) PCR analysis of the Δpld mutant. Chromosomal primers DW1194 and DW1195 were used with template DNAs isolated from R. prowazekii. Lane 1, Δpld template DNA product (2,860 bp); lane 2, wild-type template DNA product (2,326 bp). Size markers (M) are indicated in kilobase pairs. (C) Hybridization of a specific pld gene probe (lane 1, 622 bp covering the entire open reading frame of the gene) to R. prowazekii chromosomal DNA digested with PstI. Lane 2, DNA isolated from the Δpld mutant strain; lane 3, DNA isolated from the wild-type Evir strain. Molecular size markers (M) are indicated.