FIG. 5.

CD4⁺ T cells from infected caspase 1^−/− mice secrete IFN-γ in response to B. burgdorferi lysate stimulation in vitro. CD4⁺ T cells enriched from splenocytes from WT and caspase 1^−/− mice were incubated with mitomycin C-treated antigen-presenting cells and stimulated with B. burgdorferi lysate (Bb lysate). After 72 h, supernatants were collected, and the amount of IFN-γ was analyzed by ELISA. The results are reported as the means of triplicate samples plus standard errors of the means (error bars) and are representative of three separate experiments.