FIG. 3.

More efficient induction of IL-2 and IFN-γ responses by DCs from lpg2⁻ L. major-infected mice. DCs isolated from BALB/c mice infected for 3 days with WT or lpg2⁻ L. major were pulsed overnight with full-length OVA protein (100 μg/ml) and then cocultured with purified CFSE-labeled CD4⁺ T cells from DO11.10 transgenic mice. After 4 days, proliferation and IFN-γ production were determined by flow cytometry (A). The culture supernatant fluids were collected and assayed for IL-2 and IFN-γ by ELISA (B). Some purified DCs were cocultured with a LACK-specific T-cell hybridoma (LMR7.5) for 72 h, and the culture supernatant fluids were assayed for IL-2 and IFN-γ by ELISA (C). The data presented are representative of three (A and B) and two (C) independent experiments (n = 4 to 5 mice/group in panels A and B) with similar results. ND, not done. *, P < 0.05; **, P < 0.01.