FIG. 5.

The immunomodulatory effect of L. major glycoconjugate molecules is independent of site of infection. BALB/c mice infected in the ear (intradermal) with 5 million WT or lpg2⁻ L. major parasites were sacrificed after 3 days, and CD11c⁺ DCs were purified from pooled dLN cells; stained for surface expression of MHC class II, CD40, CD80, and CD86 molecules; and analyzed by flow cytometry (A). Some unfractionated dLN cells were stimulated with SLA for 3 days, and the percentage of IFN-γ- and IL-4-producing cells was determined by intracellular cytokine staining (B). The data presented are representative of two independent experiments (n = 4 to 5 mice/group) with similar results.