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. 2009 May 18;77(8):3402–3411. doi: 10.1128/IAI.00292-09

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FIG. 6. — Interaction of CpxR with the promoter region of lspB. (A) Sequence logo for the CpxR recognition site based on 24 published E. coli CpxR binding sequences (17, 67). (B) Putative CpxR-binding consensus sequence upstream from the lspB ORF. (C) EMSA using 50 pmol purified His-tagged CpxR, together with the DIG-labeled lspB promoter region DNA (nt −157 to +43; panel 1), the DIG-labeled lspA1 promoter region DNA (nt −179 to +21; panel 2), and the DIG-labeled gyrB promoter region DNA (nt −154 to +47; panel 3).