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Fluorescence microscopy with Z-stack analysis of corneal epithelial cells grown with and without Matrigel at 5 h postinfection with P. aeruginosa strain PA01 expressing GFP. Cells grown on Matrigel showed bacterial retention in two distinct zones: at the apical cell surface (A) and the filter surface (B). Cells grown without Matrigel showed apical surface retention of bacteria (C) but no filter surface retention (D). Arrows indicate filter pores.
