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. 2009 Jun 1;77(8):3302–3311. doi: 10.1128/IAI.00396-09

TABLE 4.

Comparison of gene induction following B. burgdorferi infection of C3H and ISCL mice

Probe set identification no. Gene product description Gene product designation Chromosomal location of gene Rank for gene in C3H mice (by 2.0 chip results)a Upregulation (n-fold) at 1 wk postinfectionb in:
C3H micec
C3.B6 mice (2.0 chip result)
B6.C3 mice (2.0 chip result)
B6 mice (2.0 chip result)
2.0 chip result 2.0A chip result CB-Bb2-Bb3 CB-Bb4 BC-Bb2-Bb3 BC-Bb4
1424923_at Serine (or cysteine) peptidase inhibitor, clade A Serpina3g Chr 12, E 1 45.92 6.64 8.24 55.17 NCd NC NC
1419043_a_at IFN-inducible GTPase 1 Iigp1 Chr 18, E1 2 45.53 165.31 24.03 46.97 NC 2.15 NC
1449009_at T-cell-specific GTPase Tgtp Chr 11, B1.2 3 36.81 42.31 21.01 34.30 NC 2.44 NC
1420549_at Guanylate nucleotide binding protein 1 Gbp1 Chr 3, H1 3, 67.4 cM 4 32.70 30.74 39.02 181.00 NC NC NC
1417141_at IFN-γ-induced GTPase Igtp Chr 11, B1.3 11, 32.0 cM 5 32.64 128.48 12.52 57.84 NC NC NC
1418652_ate Chemokine (C-X-C motif) ligand 9 Cxcl9 Chr 5, E2 5, 53.0 cM 6 31.53 17.14 33.08 67.86 NC 2.26 NC
1450783_at IFN-induced protein with tetratricopeptide repeats 1 Ifit1 Chr 19, C1 7 28.87 8.82 10.90 85.66 3.61 2.52 NC
1418930_at Chemokine (C-X-C motif) ligand 10 Cxcl10 Chr 5, E2 5, 53.0 cM 8 26.31 2.58 16.96 147.58 NC 3.73 NC
1423555_a_at IFN-induced protein 44 Ifi44 Chr 3, H3 9 22.10 5.72 7.75 55.32 NC NC NC
1438676_at Macrophage activation protein 2-like protein Mpa2l Chr 5, E5 10 19.36 35.91 6.63 29.38 NC NC NC
1417292_at IFN-γ-inducible protein 47 Ifi47 Chr 11, B1.2 11 18.37 6.55 8.31 20.72 NC NC NC
1417793_at IFN-inducible GTPase 2 Iigp2 Chr 11, B1.3 12 16.37 122.78 15.95 29.97 NC NC NC
1418392_a_at Guanylate nucleotide binding protein 4 Gbp4 Chr 3, H1 13 15.50 14.42 9.12 29.86 2.58 2.56 NC
1425225_at Fc fragment of immunoglobulin G, low affinity IIIa, receptor Fcgr3a Chr 1, H3 1, 92.29 cM 14 15.34 36.40 3.92 33.72 NC 2.76 NC
1419714_at CD274 antigen Cd274 Chr 19, C2 15 14.94 32.80 7.32 56.27 NC NC NC
1428007_at Keratin-associated protein 13-1 Krtap13-1 Chr 16, C3.3 16 14.89 NC NC NC NC NC NC
1421596_s_at Histocompatibility protein 28 H28 Chr 3, H3 3, 82.5 cM 17 13.81 4.80 9.41 26.86 NC NC NC
1450033_a_at Signal transducer and activator of transcription 1 Stat1 Chr 1, C1.1 1, 25.9 cM 18 12.65 22.73 12.76 31.89 3.26 NC NC
1418240_at Guanylate nucleotide binding protein 2 Gbp2 Chr 3, H1 3, 67.4 cM 19 11.41 12.15 6.95 28.35 2.25 2.14 NC
1418825_at Immunity-related GTPase family, M Irgm Chr 11, B1.2 20 11.38 38.84 8.70 18.06 NC NC NC
1416016_at Transporter 1, ATP binding cassette, subfamily B Tap1 Chr 17, B1 17, 18.6 cM 21 9.92 15.24 5.40 19.91 2.18 NC NC
a

The 21 transcripts most highly upregulated in C3H mice, according to the results of Affymetrix GeneChip 2.0 analysis, are ranked in order from that with the highest level of induction to that with the lowest. Values for the 20 transcripts also upregulated >2-fold in congenic mice with the C3H background are indicated in italics.

b

Increase in transcript levels in samples from infected mice at 1 week postinfection compared to those in samples from uninfected mice of each genotype. The gene transcripts most highly induced in the joints of C3H mice following infection with B. burgdorferi were highly upregulated in C3.B6-Bbaa2-Bbaa3 (CB-Bb2-Bb3) and C3.B6-Bbaa4 (CB-Bb4) ISCL but not in B6.C3-Bbaa2-Bbaa3 (BC-Bb2-Bb3) and B6.C3-Bbaa4 (BC-Bb4) ISCL.

c

The levels of induction (n-fold) of the top 21 transcripts upregulated in the joint tissues of C3H mice following infection with B. burgdorferi, as determined in the present study using Affymetrix GeneChip 2.0, are compared with those determined in the previous study (11) using Affymetrix GeneChip 2.0A.

d

NC, no change (indicates that transcript level was altered <2-fold compared with that in samples from uninfected mice).

e

Data in boldface correspond to genes mapping to Bbaa QTL.